Fig. 1. Generation of GIT1-deficient mice.

(A) Murine Git1 targeting vector. Protein-encoding exons are indicated by solid boxes. The lox-P sites are depicted by filled shaded triangles, and the thymidine kinase (HSV-TK), neomycin (PKG-Neo), and diphtheria toxin (PKG-DT) selection markers are indicated. Rearrangements following Cre recombinase treatment are shown as Git1^flox for the conditional allele and Git1^del for the knockout allele. F1, F2 and R1 indicate the location and orientation of genotyping primers. (B) Results from genotyping of GIT1 mice by genomic PCR. (C) Confirmation of GIT1 protein deficiency in cerebellar lysates from GIT1-KO animals by Western blot. (D) Morphology of WT and GIT1-KO brains assessed by neuronal staining using NeuN antibody.