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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Dev Cell. 2008 Aug;15(2):261–271. doi: 10.1016/j.devcel.2008.07.002

Figure 2. Cis-regulatory sequences that direct endothelial-specific expression of Egfl7/miR-126.

Figure 2

(A) E12.5 transgenic mouse embryo harboring a lacZ transgene controlled by 5.4kb 5’ flanking DNA upstream of the Egfl7/miR-126 gene. EC specific lacZ expression showing a) whole mount embryo, and sagittal section in b) perichondral region, c) dermis, d) brain, and e) outflow tract. Scale bar equals 50um.

(B) Schematic diagrams of genomic regions upstream of the Egfl7/miR-126 gene tested for regulation of lacZ in transgenic mice at E12.5. The fraction of transgenic embryos showing endothelial-specific expression of lacZ is shown. Evolutionary conservation of the Egfl7/miR-126 5’ flanking region is shown below. The conserved ETS binding sites are highlighted in pink and light blue.

(C) Genomic fragment for regulatory region 1(Region1-Luc) was tested for activation by increasing amounts of an expression plasmid encoding Ets1 or Ets1 mutant lacking the DNA binding domain (Ets1mut) in COS-7 cells. A deletion mutation was introduced into the ETS binding site (Region1(mut)-Luc). Ets1 activated Region1-Luc but not Region1(mut)-Luc, while Ets1mut failed to activate either construct.