Table 2.

RT-qPCR results for 9 day old larval midguts and non-midgut tissue (restbody) for genes identified by GeneFishing.

Gene	Midgut	ct value (Bac)	Restbody	ct value (Bac)
Defense
cathepsin L-like protease	1.77 ± 0.018	19.99	1.17 ± 0.034	19.22
cytochrome c oxidase polypeptide Vb	1.07 ± 0.074	17.81	-1.08 ± 0.094	18.61
mitochondrial cytochrome c oxidase subunit VIa	-1.02 ± 0.115	17.93	-1.15 ± 0.004	19.3
Mn superoxide dismutase	-1.03 ± 0.026	20.18	-1.75 ± 0.030	20.38
lectin 4 C-type lectin	-3.49 ± 0.048	25.53	-1.96 ± 0.129	21.9
Glutathion S-transferase 1	1.80 ± 0.037	20.35	-9.17 ± 0.058	27.94
ubiquinol-cytochrome c reductase	-1.16 ± 0.005	19.27	-1.10 ± 0.014	19.74
Development
trypsin-like serine protease	-1.35 ± 0.212	32.96	2.02 ± 0.037	23.89
hypothetical protein AaeL_AAEL012245, contains chitin binding domain	-1.29 ± 0.210	31.98	-1.42 ± 0.163	31.89
Perithrophin A	-78.25 ± 0.067	29.15	-1.28 ± 0.148	28.17
myosin I	-1.27 ± 0.014	22.73	-1.72 ± 0.035	22.47
titin1	-2.32 ± 0.018	20.1	1.29 ± 0.028	16.64
Digestion
tyrosine-protein kinase	-1.40 ± 0.134	25	3.96 ± 0.007	28.89
DNA related
C14orf124 protein	-1.03 ± 0.023	22.48	-1.15 ± 0.030	21.59
zinc finger protein	-1.05 ± 0.065	21.13	-1.08 ± 0.267	21.97
Metabolism
alcohol dehydrogenase (acceptor)	-2.05 ± 0.939	19.8	2.25 ± 0.062	22.76
protease inhibitor 1	-2.45 ± 0.325	29.81	-1.18 ± 0.131	19.04
Serine/threonine-protein kinase polo	-1.45 ± 0.097	31.06	-1.17 ± 0.048	26.2
short-chain dehydrogenase	-1.20 ± 0.018	21.09	6.12 ± 0.117	26.29
protein disulfide isomerase	-1.09 ± 0.078	17.63	1.25 ± 0.074	17.66
Signaling
putative G-protein coupled receptor	-1.40 ± 0.25	19.31	9.48 ± 0.129	25.49

Results are shown for animals grown on bacterial diet, where bacteria-free diet treatments were set to 1 (values are mean ± SD).