Figure 5.
Expression of the HsCdc6A1A2A3 mutant inhibits initiation of DNA replication. HSF8 cells expressing the indicated GFP-HsCdc6 proteins and uninfected control cells were synchronized in G0 by serum deprivation and then stimulated with 20% FCS in fresh medium in the presence of 10 μM BrdUrd. Cells were fixed and stained with anti-BrdUrd antibody and Hoechst 33258 at the indicated times. (A) Representative photomicrographs of B at 20 and 30 h are shown. (B) Percentages of BrdUrd-positive cells in indicated points are shown. Each value represents a minimum count of 100 cells.