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. 2009 Feb 18;136(7):1063–1069. doi: 10.1242/dev.030957

Fig. 3.

Fig. 3.

EpiSCs transfected with Klf4 can convert to ground state pluripotency. (A) Oct4-positive colonies obtained by transfection with Klf4 and transfer to 2i/Lif after 72 hours. Images were taken after 9 days in 2i/Lif. (B) qRT-PCR analysis of marker gene expression in ES cells, EpiSCs and derivative Epi-iPS cells isolated in 2i/Lif. y-axis, relative expression normalised to Gapdh. (C) me3H3K27 staining of female EpiSCs and derivative Epi-iPS cells. (D) Images of Epi-iPS colonies after 10 days in 2i/Lif, showing mutually exclusive expression of DsRed and Oct4-GFP. (E) Flow cytometry analysis of four expanded Epi-iPS clones. Two clones retain weak but detectable red fluorescence. (F) qRT-PCR analysis of Klf4 transgene and DsRed expression in Epi-iPS cell clones and parental EpiSC line. y-axis, relative expression normalised to Gapdh. (G) Chimeric mouse produced from the K4C12 Epi-iPS clone and agouti germline offspring.