Fig. 2.

In situ hybridization of frzb-1 and crescent mRNA during neurula and early tailbud stages. (A-H′) In situ hybridization of frzb-1 (A-D) and crescent (E-H) during neurula and early tailbud stages. frzb-1 and crescent are stained purple/blue, the cement gland marker (XCG), dark red and the CNS-specific marker (nrp1), light orange. Arrows indicate the presumptive primary mouth. cg, cement gland. A-H are frontal views, scale bars: 200 μm; A′-H′ are sagittal sections with anterior to the left, scale bars: 130 μm.