Fig. 4.

Deregulation of Wg and Notch responses at the DV border of the wing disc. (A,C,E) Expression of Sens (A), Wg (C) and Ct (E) in a wild-type wing disc. (B,B′) Sens repression (red) in a wing disc containing lin^G1 clones (lack of GFP and outlined). (D,D′) Wg is ectopically activated (green) in lin^G1 clones (red) induced in the wing pouch. (F,F′) Ct activation (green) in some lin^G1 clones in the wing pouch (red). Very large clones do not activate Ct probably because they were induced before the onset of the DV border. (G-J′) Wing discs containing transient ectopic UAS-drm clones (red) produced by the tubGal80^ts technique. After 7 hours at the restrictive temperature, Bowl protein (green) (G,G′) is stabilized. After 18 hours, Sens (H,H′) is autonomously repressed (green) and Wg (see brackets in I,I′) and Ct (J,J′) are activated. (K-K″) Ectopic clones co-expressing Lin RNAi and Bowl RNAi (green). The effects of knocking down lin by ectopic Lin RNAi are suppressed by co-expression of Bowl RNAi.