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. 2009 Jan 21;136(5):749–760. doi: 10.1242/dev.024703

Fig. 4.

Fig. 4.

Spt binds clusters of T-box sites near tbx6 and deltaD in vivo. We used qPCR to measure the enrichment of the genomic sequences highlighted in blue in B-E in DNA precipitated using a Spt antibody. (A,B) Regions of the T-box motif cluster upstream of tbx6 (B) are 7.9±1.8 and 6.2±0.39-fold enriched in Spt-bound DNA (A). A region of the intronic T-box motif cluster in the dld second intron (C) is enriched 4.3±0.95 fold in Spt-bound DNA (A). As a negative control, we determined the enrichment level of parts of the rhodopsin (rho) (D) and v-maf musculoaponeurotic fibrossarcoma oncogne protein b (mafba) (E) upstream regions in Spt-bound DNA using qPCR and saw no enrichment in Spt-bound DNA (A).