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. 2009 Feb 6;136(5):855–864. doi: 10.1242/dev.028605

Fig. 3.

Fig. 3.

Altered expression of EphA4 mRNA in Twist1 mutant suture. Probes against EphA4 (A-B′) or Twist1 (E-F′) mRNA were incubated with sections of wild-type, Twist1+/- or EphA4-/- embryos at E 14.5. Alternate sections were stained for ALP activity (C,D,G,H). Note co-expression of EphA4 and Twist1 in ectocranial mesenchyme (arrows). Also note reduced expression of EphA4 in Twist1+/- mutant but unchanged expression of Twist1 in EphA4-/- mutant. Plane of section was as in Fig. 2. fb, frontal bone; pb, parietal bone.