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. 2009 Feb 6;136(5):855–864. doi: 10.1242/dev.028605

Fig. 5.

Fig. 5.

Co-regulation of Erk1/2 phosphorylation in developing coronal suture by Twist1 and EphA4. (A-H) Heads of E14.5 embryos with the indicated genotypes were sectioned as in Fig. 2 and stained either for P-Erk1/2 or ALP. (I-L) Sections were stained for P-Erk1/2 (I,K) or total Erk (J,L). Note progressive reduction in P-Erk1/2 staining in ectocranial layer (arrows) as dosage of Twist1 and EphA4 are reduced. Note also that total Erk1/2 is unaffected in Twist1+/-; EphA4+/- embryos whereas P-Erk1/2 is substantially reduced. Thus Twist1 and EphA4 regulate Erk1/2 phosphorylation specifically. cs, coronal suture; fb, frontal bone; pb, parietal bone.