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. 2008 Dec 4;136(2):231–240. doi: 10.1242/dev.028043

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Fig. 7. — Grg5 knockdown decreases the number of Lim3^- motor columns. (A-D) St 24 chick embryos electroporated with Grg5 siRNAs. (A) The right-hand half of the spinal cord is electroporated, as indicated by GFP expression. (B) In situ hybridization of Grg5 mRNA showing efficient ablation of Grg5 mRNA (asterisk) on the Grg5 siRNA-electroporated side. (C,D) Confocal micrographs of sectioned chick spinal cords showing immunohistochemical analysis of motor column markers. (E) Bar chart showing the percentage of motor columns in electroporated Grg5 siRNAs (black bars), control siRNAs (gray bars) and contralateral non-electroporated (white bars, set as 100%) sides of the spinal cord at forelimb regions. n=4-5 embryos, mean ± s.e.m.