Figure 9. Effect on splenocyte survival of diverse pretreatments applied to the non-cytosolic trypanosome fraction (NCF).

Splenocytes were exposed for 75 min to the non-cytosolic fraction corresponding to 4 10⁸ trypanosome equivalents per ml. Mock, PBS; NCF 37°C and 4°C, non-cytosolic fraction kept at 37 or 4°C respectively for 24 h prior to incorporation onto the cells; Pf, E64 and PK, treatment of NCF with Pefablock or E64 or proteinase K respectively for 24 hours prior to incorporation onto cells. The three cell subsets represented are normocytic and annexinV/PI double negative (red, healthy), normocytic and annexinV/PI double positive (green, early necrosis), and microcytic and annexinV/PI double positive (blue, late necrosis). The values presented are means of three independent experiments.