Table 1. Parasite load, lymphogram, and lymphocyte death during the first parasitaemia wave.
Time | 1 h bi | 52 h pi | 64 h pi | 72 h pi | 84 h pi | 92 h pi | ||||||
CTL | PRINC. | CTL | PRINC. | CTL | PRINC. | CTL | PRINC. | CTL | PRINC. | CTL | PRINC. | |
Parasite load (×107/ml) | 0 | 0 | 0 | 0.23±0.57 | 0 | 1.07±0.20 | 0 | 4.17±0.65 | 0 | 12.6±1.78 | 0 | 28.9±1.96 |
Lymphocytes (×106/ml) | 5.16±0.24 | 5.01±0.15 | 5.02±0.21 | 5.83±0.21* | 5.23±0.17 | 4.72±0.30* | 4.99±0.24 | 2.80±0.40* | 5.41±0.26 | 1.13±0.29* | 4.88±0.24 | 0.83±0.26* |
CD4+ (×106/ml) | 1.25±0.18 | 1.24±0.14 | 1.12±0.12 | 1.00±0.15 | 1.11±0.19 | 0.83±0.14* | 1.17±0.13 | 0.63±0.10* | 1.16±0.19 | 0.24±0.04* | 1.10±0.14 | 0.19±0.05* |
CD8+ (×106/ml) | 0.69±0.08 | 0.67±0.08 | 0.67±0.06 | 0.62±0.06 | 0.67±0.08 | 0.45±0.06* | 0.63±0.08 | 0.25±0.02* | 0.58±0.08 | 0.11±0.02* | 0.58±0.06 | 0.08±0.01* |
CD19+ (×106/ml) | 3.22±0.26 | 3.10±0.21 | 3.23±0.20 | 4.20±0.20* | 3.45±0.25 | 3.45±0.19 | 3.19±0.16 | 1.92±0.10* | 3.67±0.25 | 0.78±0.06* | 3.20±0.18 | 0.56±0.07* |
CD4+ (%) | 24.3±3.5 | 24.7±2.9 | 22.2±2.4 | 17.2±2.6* | 21.2±3.6 | 17.5±2.9* | 23.4±2.6 | 22.6±3.5 | 21.4±3.4 | 21.2±3.5 | 22.5±2.8 | 22.5±6.1 |
CD8+ (%) | 13.4±1.6 | 13.3±1.5 | 13.4±1.2 | 10.7±1.0* | 12.8±1.6 | 9.5±1.3* | 12.7±1.7 | 8.8±0.9* | 10.7±1.5 | 9.4±2.1 | 12.0±1.3 | 10.1±1.8* |
CD19+ (%) | 62.3±5.0 | 61.9±4.1 | 64.4±3.3 | 72.1±3.5* | 66.0±4.8 | 73.0±4.1* | 64.0±3.1 | 68.5±3.7* | 67.9±4.7 | 69.5±5.4 | 65.5±3.6 | 67.5±8.0 |
Apoptotic CD4 (%) | 5.41±1.35 | 5.12±1.6 | 5.64±1.33 | 6.12±1.44 | 4.56±1.04 | 6.74±1.51 | 4.13±0.82 | 5.19±1.23 | 3.25±0.86 | 5.07±1.34 | 5.74±0.86 | 5.28±1.34 |
Apoptotic CD8 (%) | 1.40±0.47 | 1.70±0.87 | 1.54±0.58 | 2.40±1.69 | 1.56±0.43 | 1.73±1.23 | 1.10±0.28 | 1.15±0.44 | 1.05±0.51 | 1.11±0.59 | 1.32±0.51 | 1.45±0.59 |
Apoptotic CD19 (%) | 0.39±0.23 | 0.67±0.37 | 0.76±0.36 | 0.69±0.39 | 0.53±0.34 | 0.62±0.29 | 0.41±0.14 | 0.56±0.27 | 0.48±0.18 | 0.45±0.30 | 0.54±0.18 | 0.48±0.30 |
Necrotic CD4 (%) | 0.5±0.29 | 0.6±0.22 | 0.4±0.09 | 0.5±0.09 | 0.4±0.11 | 0.4±0.15 | 0.3±0.09 | 0.4±0.08 | 0.35±0.19 | 0.4±0.18 | 0.4±0.18 | 0.5±0.11 |
Necrotic CD8 (%) | 0.5±0.44 | 0.5±0.17 | 0.3±0.17 | 0.2±0.07 | 0.1±0.03 | 0.2±0.11 | 0.3±0.13 | 0.5±0.30 | 0.2±0.10 | 0.3±0.10 | 0.2±0.12 | 0.5±0.28 |
Necrotic CD19 (%) | 2.0±2.05 | 1.8±1.02 | 2.2±0.83 | 2.0±0.81 | 3.9±2.61 | 1.5±0.75 | 2.0±0.69 | 1.5±0.82 | 2.5±0.94 | 1.9±0.64 | 2.6±1.08 | 1.69±0.81 |
Results are means±SD. CTL, control mice; PRINC., principal mice inoculated with T. evansi; bi, before inoculation; pi, post-inoculation. Asterisks show values significantly different from corresponding isotime values among control mice (p<0.05).