Table 5. Nucleotide (nt) and amino acid (aa) changes in sequences of Huh-7 – passaged IQT-1950 strain DENV relative to uncloned parent DENV.

Virus	Passagea	nt position	Region	nt change	aa change
IQT-1950	P10 – A	506	prM	T→Y (T/C)	-
IQT-1950	P10 – B Alt	978	ENV	G→S (G/C)	(V→L)
IQT-1950	P10 – A	1119	ENV	G→A	E→K
IQT-1950	P10 – B	1121	ENV	G→A	-
IQT-1950	P10 – A Alt	1121	ENV	G→R (G/A)	-
IQT-1950	P10 – A Alt	1412	ENV	T→Y (T/C)	-
IQT-1950	P10 – A	1539	ENV	G→R (G/A)	(E→K)
IQT-1950	P10 – B	1539	ENV	G→A	E→K
IQT-1950	P10 – A Alt	1539	ENV	G→A	E→K
IQT-1950	P10 – B Alt	1539	ENV	G→A	E→K
IQT-1950	P10 – B	1657	ENV	A→G	-
IQT-1950	P10 – B Alt	2014	ENV	A→G	E→G
IQT-1950	P10 – B Alt	4472	NS2B	A→R (A/G)	(I→M)
IQT-1950	P10 – A	5031	NS3	G→R (G/A)	(G→S)
IQT-1950	P10 – B	7083	NS4B	A→W (A/T)	(M→L)
IQT-1950	P10 – B Alt	7409	NS4B	T→C	-
IQT-1950	P10 – B	7505	NS4B	G→T	-
IQT-1950	P10 – A Alt	7505	NS4B	G→K (G/T)	-
IQT-1950	P10 – B Alt	7514	NS4B	T→G	-
IQT-1950	P10 – A	7775	NS5	T→Y (T/C)	-
IQT-1950	P10 – B Alt	8117	NS5	T→C	-
IQT-1950	P10 – B Alt	10362	3′- UTR	A→G	-
IQT-1950	P10 – B	10392	3′- UTR	T→C	-
IQT-1950	P10 – A Alt	10392	3′- UTR	T→C	-
IQT-1950	P10 – B Alt	10392	3′- UTR	T→C	-

^aEach passage was performed in duplicate (series A and B); P10 = passage 10; Alt = alternate passage; - = no change.