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. 2009 Jun 5;5(6):e1000468. doi: 10.1371/journal.ppat.1000468

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Hanrahan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Each of the panels, A–E, contains a merged image of the nucleus and kinetoplast, together with the GPI-PLC probed with anti-GPI-PLC antibody (magenta) and the phase image; (panel A), wild-type GPI-PLC^+/+ bloodstream form trypanosome; (panel B), a GPI-PLC^−/− null mutant bloodstream form trypanosome; (panel C), a wild-type procyclic form trypanosome; (panel D), a low power view of wild-type GPI-PLC^+/+ bloodstream form trypanosomes and (panel E), a low power view of GPI-PLC^−/− null mutant bloodstream form trypanosomes. The phase image (panel F) of S-42 stumpy bloodstream forms shows the typical morphology of these forms in this strain, while the fluorescence image (panel G) shows the location of the GPI-PLC (magenta) and the merge of these two images shows their spatial relationship (panel H). The location of YFP-tagged GPI-PLC is shown in the composite image of six bloodstream forms in panel I and the arrowheads point to the endo-membrane-bound spaces containing a small amount of GPI-PLC. Bars, 5 µm.