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. 2009 Jun 5;5(6):e1000468. doi: 10.1371/journal.ppat.1000468

Figure 5. Effect of temperature and detergent on the detection of the VSG, tubulin, ISG-70 and GPI-PLC.

Figure 5

Panel A shows the detection of VSG, tubulin, ISG-70 and GPI-PLC in wild type bloodstream form trypanosomes fixed at 0°C in the presence of triton X-100. Panel B shows the detection of VSG, tubulin, ISG-70 and the GPI-PLC in wild type bloodstream form trypanosomes fixed at 0°C in the absence of triton X-100. Panel C shows the detection of VSG, tubulin, ISG-70 and GPI-PLC in wild type bloodstream form trypanosomes fixed at 37°C in the presence of Tx-100. Panel D shows the detection of VSG, tubulin, ISG-70 and GPI-PLC in wild type bloodstream form trypanosomes fixed at 37°C in the absence of triton X-100. The inset in each panel is the Differential Interference contrast (DIC) image. Bars, 5 µm.