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. 2009 Jun 1;4(6):e5755. doi: 10.1371/journal.pone.0005755

Figure 7. C1q is expressed in normal archival prostate tissue samples and is significantly downregulated in benign prostatic hyperplasia (BPH) and prostate cancer.

Figure 7

(A) Prostate tissues, including normal prostate, BPH and prostate cancer, were stained with specific antibody against C1q and then with secondary fluorescent antibody. Nuclei were stained with DAPI (100× magnification). (B) C1q is significantly downregulated in BPH and prostate cancer, as compared to age-matched prostate tissues (p<0.0001, n = 5; Student's t test). When non-immune serum was used as the source for primary antibody, no signal was observed (data not shown). (C) C1q is expressed in the basal and epithelial cells of the archival normal prostate glands (400× magnification). Both C1q and p-WOX1 are colocalized in these cells. Nuclei were stained with DAPI. Similar results were observed with the archival gland cells from BPH. Compared to the normal prostate tissues, the exposure time for taking the pictures for BPH was increased. Scale bar, 100 µm.