Skip to main content
. 2009 Jun 1;4(6):e5755. doi: 10.1371/journal.pone.0005755

Figure 10. Serum C1q and C6 restrict constitutive JNK1 activation but have no effect on STAT3.

Figure 10

(A) DU145 cells were cultured under serum-free (SF) conditions, or in 1% NHS, ΔC1q or ΔC6 serum for 16–24 hr. In the absence of C1q and C6, spontaneous JNK1 activation occurred (versus SF controls; p<0.001; Student's t test), as determined by Western blotting. A representative set of data is shown from 3 experiments. (B) Similar results were observed by immunofluorescence microscopy, which shows significant JNK1 activation or nuclear accumulation under serum C1q- and C6-free conditions (versus SF; p<0.001; Student's t test, n = 3). ΔC9 serum had no effect. Approximately 200 cells were counted from each experiment. (C,D) There was no STAT3 activation in DU145 cells under all the indicated serum conditions. Under C1q-free conditions, HA (50 µg/ml) effectively induced STAT3 phosphorylation in cells during treatment for 1 hr. Approximately 200 cells were counted from each experiment (n = 3).