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. 2009 Jun 3;4(6):e5785. doi: 10.1371/journal.pone.0005785

Figure 4. Modified setup for the measurement of the cross-section of the illumination beam.

Figure 4

The illumination beam was focused towards the microscope and a fluorescent cover slip was placed in its way. By observing the fluorescence of the cover slip, the cross-sections of the illumination beam was measured. By moving the cylindrical lens, cross-sections of various positions from the focal point can be observed.