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. 1999 May 25;96(11):6205–6210. doi: 10.1073/pnas.96.11.6205

Figure 1.

Figure 1

An Spc110p-containing SPB subcomplex. (A) An Spc110p-containing SPB subcomplex was purified from cells with a protein A-tagged SPC110 (ProA–SPC110) (lane 2) by using IgG Sepharose. The protein bands were subjected to matrix-assisted laser desorption ionization (MALDI) MS analysis. Wild-type cells, containing Spc110p without a ProA tag (lane 1), were treated identically to identify proteins that bound nonspecifically to the IgG resin. The major proteins are encoded by SPC110 (band 1, ProA–Spc110p homodimer; band 2, ProA-Spc110p; bands 3 and 4, ProA–Spc110p degradation products), SPC42 (band 5), SPC29 (band 6), and CMD1 (band 7). (B) Coimmunoprecipitation of Spc29p and Spc110p. High-salt extracts from cells expressing SPC110 SPC29–3HA (lane 1) or ProA–SPC110 SPC29–3HA (lane 2) were incubated with IgG Sepharose. The immunoblots were probed with the indicated antibodies.