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. 1999 May 25;96(11):6205–6210. doi: 10.1073/pnas.96.11.6205

Figure 3.

Figure 3

Cellular localization of Spc29p and Spc42p. (A) Localization of Spc29p-GFP after its overexpression from the Gal1 promoter for 3 hours at 30°C was determined by using fluorescence microscopy. DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI). (B and C) Electron microscopic picture of an SPB of mps2-1 cells with (C) or without (B) overexpression of SPC42. Cells of mps2-1 with or without Gal1–SPC42 were shifted to 37°C for 30 min in 2% raffinose medium followed by the induction of the Gal1 promoter by the addition of 2% galactose. ∗ in C indicates the Spc42p polymer. (D and E) Electron microscopic picture of an SPB from cells with (E) and without (D) cooverexpression of SPC29, SPC42, and SPC110. Cmd1p is probably present in excess and was therefore not overexpressed. ∗ in E indicates the Spc42p polymer. C, central plaque; Cy, cytoplasm; HB, half bridge; I, inner plaque; NE, nuclear envelope; Nu, nucleus; O, outer plaque; S, satellite. (Bar = 0.25 μm.). B–E are all of same magnification.