Figure 6.
Model for the functions of Spc29p. Kar1p, and Cdc31p are components of half bridge (5, 7) that function in SPB duplication (4, 6). Spc72p anchors the yeast γ-tubulin complex containing Tub4p, Spc98p, and Spc97p to the outer plaque (11). The yeast γ-tubulin complex is bound to the inner plaque via Spc110p (20). Spc42p forms a layer within the SPB (2). Cnm67p and Spc94p/Nud1p are components of the cytoplasmic outer plaque (26). Based on our results, we propose that Cnm67p binds to Spc42p and Spc94p/Nud1p interacts with Cnm67p. Spc29p is bridging C–Spc110p and N–Spc42p. During SPB duplication, Spc29p, N–Spc42p, and C–Spc110p form a critical border of cytoplasmic and nuclear assembled SPB subcomplexes. Spc42p may also be part of the satellite that forms in G1 phase of the cell cycle (3).