Table 1.
Multicopy suppression analysis
| Mutant | Multicopy gene | Temperature, °C
|
||||
|---|---|---|---|---|---|---|
| 23 | 30 | 33 | 35 | 37 | ||
| spc29-2 | SPC29 | +++ | +++ | +++ | +++ | +++ |
| SPC42 | +++ | +++ | +++ | − | − | |
| SPC110 | +++ | +++ | +++ | − | − | |
| CDC31 | +++ | +++ | +++ | +++ | +++ | |
| KAR1 | +++ | +++ | − | − | ||
| control | +++ | +++ | +++ | − | − | |
| spc29-3 | SPC29 | +++ | +++ | +++ | +++ | +++ |
| SPC42 | +++ | +++ | − | − | − | |
| SPC110 | +++ | + | − | − | − | |
| CDC31 | +++ | +++ | − | − | − | |
| KAR1 | +++ | − | − | − | − | |
| control | +++ | +++ | − | − | − | |
| spc29-9 | SPC29 | +++ | +++ | +++ | +++ | +++ |
| SPC42 | +++ | + | − | − | − | |
| SPC110 | +++ | + | − | − | − | |
| CDC31 | +++ | +++ | − | − | − | |
| KAR1 | +++ | +++ | − | − | − | |
| control | +++ | +++ | − | − | − | |
| spc42-9 | SPC42 | +++ | +++ | +++ | +++ | +++ |
| SPC29 | +++ | +++ | +++ | +++ | − | |
| SPC110 | +++ | +++ | +++ | +++ | − | |
| control | +++ | +++ | +++ | +++ | − | |
| spc42-10 | SPC42 | +++ | +++ | +++ | +++ | +++ |
| SPC29 | +++ | +++ | +++ | +++ | +++ | |
| SPC110 | +++ | +++ | +++ | − | − | |
| control | +++ | +++ | +++ | − | − | |
| spc110-118 | SPC110 | +++ | +++ | +++ | +++ | +++ |
| SPC29 | +++ | +++ | + | − | − | |
| CMDI | +++ | +++ | +++ | +++ | +++ | |
| control | +++ | +++ | +++ | + | − | |
| spc110-124 | SPC110 | +++ | +++ | +++ | +++ | +++ |
| SPC29 | +++ | +++ | +++ | − | − | |
| CMDI | +++ | +++ | +++ | +++ | +++ | |
| control | +++ | +++ | +++ | +++ | − | |
The conditional-lethal mutants spc29-2, spc29-3, spc29-9, spc42-9, spc42-10, spc110-118, and spc110-124 were transformed with either an empty 2-μm plasmid (control) or with a 2-μm plasmid carrying one of the indicated genes. Only KAR1 was on the centromere-based plasmid pRS315. Transformants were tested for growth at various temperatures. +++, good growth, +, reduced growth, −, no growth.