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Chlamydia inhibits host cell motility. The ability of HeLa cells to migrate in response to injury was tested using an in vitro scratch wound assay.28 HeLa cell monolayers were either untreated or infected with C. trachomatis LGV-L2 serovar for 12 h with various indicated multiplicity of infection (MOI) and a scratch wound was made and imaged over a period of 20 h (A). The wound gap was measured every 6 h and is expressed as percent of 0 h (B).
