Fig. 2.

Influence of DSP-4 and 5,7-DHT treatment on the expression of the Shh cascade in the rat brain. Rats received treatment with vehicle (VEH), DSP-4 or 5,7-DHT in separate experiments, and levels of Shh, Smo and Ptc mRNA were determined with in situ hybridization and quantitative densitometry. DSP-4 or 5,7-DHT treatment did not influence Shh mRNA expression in the Horizontal limb/Vertical limb of the Diagonal Band (VDB) or the expression of the Shh receptors, Smo and Ptc in the hippocampal dentate gyrus (DG) subfield. Representative autoradiographs of Shh, Smo and Ptc mRNA from vehicle and DSP-4 (Upper Panel), and vehicle and 5,7-DHT (Lower Panel) treated animals are shown. Results are expressed as percent of vehicle and are the mean ±S.E.M. (n = 5/group).