Figure 1. Expression of YFP-mPXR₄₃₁ in the livers of intact mice.

A. Following the hydrodynamic injection of YFP-mPXR₄₃₁ expression construct, fluorescence patterns were evident across liver sections of control (i) and ligand (PCN) (ii) treated mice that differed from those of YFP expression alone. B. Immunoprecipitation of YFP-mPXR₄₃₁ from mouse liver samples indicated a protein band corresponding to the predicted molecular weight of the YFP/PXR fusion protein. C. Subcellular distribution patterns of YFP-mPXR₄₃₁ expression were categorised into 3 distinct categories. An exclusively nuclear (N), where the fluorescence was confined to the nucleus, a predominantly nuclear (N>C), where a predominant nuclear localisation was evident with some cytoplasmic fluorescence, and an equal distribution (N=C), where nuclear and cytoplasmic fluorescence was evenly distributed across the cell. Nuclei are indicated by blue colour, following Hoechst 33250 staining.