Table 1.
The effect of various CaVβ subunit constructs on biophysical parameters of CaV2.2/α2δ-2 calcium channel currents expressed in tsA-201 cells
| β species | Peak IBa, pA/pF (n) | Voltage for peak IBa (mV) | Steady-state inactivation V50 inact, mV (n) |
|---|---|---|---|
| No β | −8.3 ± 1.0 (9) | +30 | −25.4 ± 7.5 (3) |
| β2a | −145.9 ± 34.9 (14)** | +20 | +0.47 ± 2.16 (6)** |
| C3,4S-β2a | −96.6 ± 12.0 (21)* | +20 | −47.6 ± 1.4 (3)** †† |
| β2-GK (226–442) | −79.9 ± 23.9 (17)* | +20 | −54.8 ± 3.0 (5)** †† |
| β2a-GK (136–442) | −63.2 ± 12.7 (18)† | +20 | −45.5 ± 3.0 (5)** †† |
| β2a-ΔvHOOK | −131.3 ± 26.8 (16)** | +20 | −14.2 ± 6.0 (6) |
| C3,4S-β2a-ΔvHOOK | −101.4 ± 15.4 (20)* | +20 | −46.0 ± 1.8 (5)** †† |
| β2-SH3 (1–135)+β2-GK (226–442) | −28.0 ± 11.1 (9)†† | +20 | −57.3 ± 1.4 (4)** †† |
Statistical significances (one-way analysis of variance and Bonferroni’s post hoc test) were determined for differences compared to CaV2.2 expressed without any β subunit or compared to CaV2.2 expressed with wild-type β2a subunit
*P < 0.05 and **P < 0.01, CaV2.2 expressed without any β subunit; †P < 0.05 and ††P < 0.01, CaV2.2 expressed with wild-type β2a subunit