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. 2009 Jun 1;330(1):21–31. doi: 10.1016/j.ydbio.2009.02.035

Fig. 4.

Fig. 4

Aphidicolin treatment and inhibition of FGF signalling do not affect the periodicity of Lfng oscillations. Chick explants cultured in the presence or absence of 50 μM aphidicolin for 3.5 h and analysed for BrdU incorporation or cLfng expression. (A) Control half explant showing BrdU incorporation throughout the explant. (B) Aphidicolin treated explant showing no BrdU incorporation. (C) Pair of explants showing the same phase of cLfng expression in the presence and absence of aphidicolin. (D, E) Chick explant pairs cultured in 25 μM SU5402 or control media supplemented with DMSO. (D) SU5402 treatment led to loss of cSprouty2 in the PSM. (E) Expression of cLfng was unaffected. (F, G) Mouse explant pairs cultured for 3 h 50 min in 25 μM SU5402 or control media supplemented with DMSO. (F) SU5402 treatment led to loss of mSprouty2 in the PSM. (G) Expression of mLfng was unaffected. “+” indicates treated half and dashed line indicates control half.