Table 1.
Calibration data to determine the lowest effective dose for the Notch signalling inhibitor DAPT, the Wnt signalling inhibitor CKI-7, the FGF signalling inhibitor SU5402 as judged by effective and reproducible loss of target gene expression in the PSM.
| DAPT | 0.1 μM | 1 μM | 10 μM | 100 μM |
|---|---|---|---|---|
| cLfng | No effect (5) | Weak phase 3 domain (5) | Weak phase 3 domain (3) | No expression in PSM (25) |
| Weak phase 3 domain (1) | No expression in PSM (1) | No expression in PSM (4) | ||
| CKI-7 | 100 μM | 150 μM | 200 μM | 400 μM |
| cLef1 | No effect (11) | Severe down regulation (23) | Severe down regulation (10) | |
| Strong down regulation (5) | No effect (1) | |||
| Weak down regulation (6) | ||||
| mLef1 | Severe down regulation (9) | weak down regulation (3) | Severe down regulation (11) | |
| No effect (6) | No effect (3) | |||
| SU5402 | 12.5 μM | 25 μM | 50 μM | 100 μM |
| cSprouty2 | Some weak expression (4) | No expression (8) | No expression (4) | No expression (4) |
| No expression (1) | ||||