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. 2009 Jun 5;4(6):e5814. doi: 10.1371/journal.pone.0005814

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Ushizawa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Expression of SOLD1 mRNA in various bovine tissues, including heart, liver, lung, spleen, and kidney, was analyzed by RT-PCR. Cotyledonary tissue at Day 150 of gestation was used as a bovine placental sample. GAPDH expression in each tissue is presented as a positive control.CSH1 expression in each tissue except for the placenta is presented as a negative control (NC). ALB expression in the other tissue is presented as a negative control (NC).(B)Quantitative expression of SOLD1 in bovine placental tissues during the initial to rate stage of pregnancy by qRT-PCR analysis. Pre-Im, pre-implantaion; Peri-Im, peri-implantation; and Post-Im, post-implantation. CON, conceptus; EEM, extra-embryonic membrane; EMB, embryo; COT, cotyledon; and ICOT, intercotyledon. D, day after insemination. Expression of these mRNAs was normalized to the expression of GAPDH measured in the corresponding RNA preparation. Values are means±SEM. Values with different letters (a, b, c and d) are significantly different (P<0.05).