Figure 2. Flow cytometry analysis showing increased proportions of CD4+CD25hi T cells and CD4+CD25+FoxP3+ regulatory T cells in peripheral blood of patients with strongyloidiasis and HTLV-1 co-infection.
Representative flow cytometry analysis of peripheral blood mononuclear cells comparing a patient with strongyloidiasis without HTLV-1 co-infection (left) to strongylioidiasis/HTLV-1 co-infected patient (right). Upper figures show CD4 and CD25 staining of cells within the lymphocyte gate. Note population of CD4+CD25hi cells in the HTLV-1 co-infected patient suggesting regulatory T cell phenotype. Lower figures show CD4+ lymphocytes stained with CD25 and intracellular staining with FoxP3 confirming that CD4+CD25hi cells are indeed FoxP3+ (regulatory T cells).
