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. 2009 Jun 5;4(6):e5820. doi: 10.1371/journal.pone.0005820

Figure 3. DTH and splenocyte proliferation in BALB/c mice vaccinated with cationic liposomal antigens before and after challenge infection.

Figure 3

Ten days after the last vaccination and 3 months after challenge infection DTH (A) and splenocyte proliferative (B) responses were measured. DTH response is expressed as the difference (in millimeters) between the thickness of the test (LAg-injected) and control (PBS-injected) footpads at 24 h. Spleens were collected and splenocytes were restimulated in vitro for 72 h with 2.5 µg/ml of specific antigens. Antigen-specific splenocyte proliferation was determined by thymidine incorporation and expressed as counts per minute. Each sample was examined in triplicate. The results are shown as the mean±S.E. for five individual mice per group, representative of two independent experiments with similar results. # p<0.0001 in comparison to control groups and * p<0.001 in comparison to data before infection as assessed by two-way ANOVA and Bonferroni's correction.