Skip to main content
. 2009 Jun 5;4(6):e5820. doi: 10.1371/journal.pone.0005820

Figure 4. Cytokine responses in BALB/c mice vaccinated with cationic liposomal antigens before and after challenge infection.

Figure 4

Ten days after the last vaccination and 3 months after challenge infection spleens were collected and splenocytes were restimulated in vitro with 2.5 µg/ml of specific antigens. After 72 h supernatants were collected and assayed for IFN-γ (A), IL-12 (B), IL-4 (C) and IL-10 (D) levels by ELISA. Each sample was examined in duplicate. The results are shown as the mean±S.E. for five individual mice per group, representative of two independent experiments with similar results. # p<0.0001 in comparison to control groups and * p<0.001 in comparison to data before infection as assessed by two-way ANOVA and Bonferroni's correction.