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. Author manuscript; available in PMC: 2009 May 26.
Published in final edited form as: Mol Microbiol. 2001 Jan;39(2):272–285. doi: 10.1046/j.1365-2958.2001.02252.x

Fig. 2. COXVI expression in transgenic procyclic trypanosomes.

Fig. 2

A. The trypanosome expression vector pHD451 engineered to express the T. brucei COXVI gene. The gene has been modified by the incorporation of a TY epitope tag immediately before the COXVI stop codon, generating the construct COXVI-TY-C.

B. Expression of COXVI-TY-C in transgenic trypanosomes under the control of tetracycline. Left: a Western blot of either wild-type procyclic trypanosomes (WT) or cells transfected with COXVI-TY-C and cultured in either the absence (U) or the presence (I) of tetracycline. The blot was probed using the anti-tag antibody BB2. Notice that an additional band is detected at approximately twice the size of the COXVI protein when using the BB2 antibody. This band, which is of variable intensity in different experiments, may represent a dimer of transgenic COXVI. Right: the same samples probed with an antibody raised to a T. brucei COXVI fusion protein.