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. Author manuscript; available in PMC: 2009 May 26.
Published in final edited form as: Mol Microbiol. 2001 Jan;39(2):272–285. doi: 10.1046/j.1365-2958.2001.02252.x

Fig. 5. Expression of COXVI mutant constructs and in vivo import of COXVI into the mitochondrion.

Fig. 5

A. Mutations of COXVI. Three COXVI mutants were generated, which either have the TY tag inserted at the N-terminus of the protein (N-TY-COXVI) or have N-terminal deletions of either seven amino acids (N-7 COXVI TY-C) or 15 amino acids (N-15-COXVI TY C). The N-terminal sequence of each predicted protein is shown for each construct.

B. Expression of COXVI-tagged mutants in procyclic-form trypanosomes detected with the anti-TY tag antibody BB2. Notice that the N-tagged protein runs slightly larger than anticipated. The N-7 and N-15 truncations run at the expected size.

C. Mitochondrial localization of tagged and truncated forms of COXVI. Purified mitochondrial vesicles were analysed directly (M lane) or subjected to either proteinase K treatment (+PK) or Triton X-100 and proteinase K treatment (+T, +PK). The respective fractions were probed with the anti-tag antibody BB2, the anti-COXVI antibody or anti-HSP60.