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. Author manuscript; available in PMC: 2009 May 26.
Published in final edited form as: Mol Microbiol. 2001 Jan;39(2):272–285. doi: 10.1046/j.1365-2958.2001.02252.x

Fig. 6. Expression of COXVI-TY-C in bloodstream-form cells.

Fig. 6

A. Northern blot of either wild-type bloodstream-form trypanosomes or bloodstream-form trypanosomes transfected with the control construct (‘control’ cell line) or with the COXVI-TY-C construct (‘COXVI-TY-C cell line’) and grown in the presence (I, induced) or absence (U, uninduced) of tetracycline. The left hand blot was probed with a riboprobe detecting COXVI mRNA; the right hand blot was hybridized with a probe recognizing the control mRNA. Left: the transgenic mRNA is larger than the endogenous COXVI mRNA. Right: the transgenic mRNA is smaller than the endogenous control transcript. These differences reflect the differing lengths of the 3′ UTRs for either the COXVI or the control transcript.

B. Protein samples from the same cell lines (‘WT’, wild-type bloodstream forms; ‘cox’, COXVI-TY-C-transfected bloodstream forms or ‘con’, bloodstream forms transfected with the control construct) detected with the anti-TY antibody BB2 (top) or with an antibody to alpha-tubulin (Sherwin et al., 1987). A signal is detected with BB2 only from the trypanosomes transfected with the control construct in the presence of tetracycline.