Table 3.

Comparison of staining intensity and percentage of melanoma cells stained with HMW-MAA and MART-1 mAbs

A. In nodal macrometastases*
Staining intensity	HMW-MAA	MART-1
Strong (+++)	11† (21%)	1 (2%)
Intermediate (++)	28 (54%)	20 (38%)
Weak (+)	13 (25%)	22 (42%)
Negative (−)	0 (0%)	9 (17%)‡
B. Comparison of the percentage of melanoma cells stained in nodal macrometastases§
Melanoma cells (%)	HMW-MAA	MART-1
100	23|| (44%)	12 (23%)
99–75	17 (33%)	7 (13%)
74–50	7 (13%)	9 (17%)
49–25	2 (4%)	8 (15%)
24–1	3 (6%)	7 (13%)
0 (negative)	0 (0)	9 (17%)‡
>50%	47 (90%)	28 (54%)
C. Comparison of the staining intensity of melanoma cells stained in nodal micrometastases¶
Staining intensity	HMW-MAA	MART-1
Strong (+++)	5**	3 (9%)
Intermediate (++)	19 (59%)	13 (41%)
Weak (+)	8 (25%)	6 (19%)
Negative (−)	0	10 (31%)‡
D. Comparison of the percentage of melanoma cells stained in nodal micrometastases††
Melanoma Cells (%)	HMW-MAA	MART-1
100	9‡‡ (28%)	3 (9%)
99–75	12 (38%)	7 (22%)
74–50	8 (25%)	6 (19%)
49–25	2 (6%)	4 (13%)
24–1	1 (3%)	2 (6%)
0 (negative)	0	10 (31%)§§
>50	29 (91%)	16 (50%)

^*n = 52 Nodes were analyzed.

^†Number of nodes with the indicated phenotype.

^‡P < 0.0001.

^§n = 52 (SLN and non-SLN) were analyzed.

^||Number of nodes with the indicated phenotype.

^¶n = 32 SLNs were analyzed.

^**Number of nodes with the indicated phenotype.

^††n = 32 SLNs were analyzed.

^‡‡Number of nodes with the indicated phenotype.

^§§P = 0.004.