Skip to main content
. 2009 Jun 12;5(6):e1000510. doi: 10.1371/journal.pgen.1000510

Figure 4. SUP-35 negatively regulates pha-1.

Figure 4

(A) Testable model for the regulation of PHA-1 by SUP-35. (B,C) Quantification of endogenous pha-1 mRNA levels in embryos by qRT-PCR in sup-35(tm1810) single mutants (B) or sup-35(e2223) pha-1(e2123 double mutants (C) using act-1 (black bar) and ama-1 (gray bar) as loading controls. Fold changes were obtained after normalizing to wild type (B) or pha-1(e2123) single mutants (C). Error bars represent s.e.m. Means of the indicated groups were analyzed for significance using a two-tailed Student's t-test (*p<0.0). Quantification of PHA-1::GFP (D) and Ppha-1::GFP (E) fluorescence in wild-type and sup-35(tm1810)mutants. The average mean GFP intensity for each genotype was analyzed for significance using a two-tailed Student's t-test (*p<0.0001). (F–I) Representative GFP (F,H) and corresponding DIC (G,I) images of PHA-1::GFP expression in wild type (F,G) and sup-35(tm1810)mutants (H,I). Digital camera exposure times were identical for all embryos assayed. Mean GFP intensities were determined as described in Materials and Methods. Scale bar in F, 10 µm for F–I.