Table 1. Expression levels of mitochondrial fission and fusion factors in Pink1 −/− tissue.
Taqman® assay | mitochondrial function | gene symbol | liver 2 days | brain 2 days | liver 18 months | brain 18 months |
Mm00612599_m1 | fusion | MfnI | ns | ns | ns | ns |
Mm00500129_m1 | fusion | MfnII | ns | ns | ns | ns |
Mm00453879_m1 | fusion | OpaI | ns | ns | ns | ns |
Mm00481580_m1 | fission | FisI | ns | ns | ns | ns |
Mm01342903_m1 | fission | DrpI | ns | ns | ns | ns |
Mm00466042_m1 | fission | Mtp18 | red. 25%; p = 0.04 | red. 10%; p = 0.09 | red. 30%; p = 0.0002 | ns |
A selective reduction of the fission factor Mtp18 mRNA levels in qPCR analysis was consistently significant at ages 2 days and 18 months for the particularly mitochondria-rich and homogeneous liver tissue, while the effect missed significance for the mixed tissue brain.
red. = reduced by factor; ns = non-significant.