Figure 4. Chromatin immunoprecipitation (ChIP) assays indicate that N-Myc directly binds lif and klf4 in neuroblastoma.

ChIP was conducted on Tet21N cells treated daily (3 days) or untreated with Tet. IgG was included as a control along with a 1∶50 and 1∶200 dilutions of input. The ChIP'd lif regions a, b, and c are represented by the black bars in Fig. 1. IgG and Input samples were run in parallel as controls.