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. 2009 Jun 3;4(6):e5778. doi: 10.1371/journal.pone.0005778

Figure 5. Shared somatic regulation between the EBV DBP BALF-2 protein gene and RAG.

Figure 5

As shown, a 200 NT 5′ region immediately adjacent to EBV BALF-2 ORF AUG start codon contains putative regulatory sequences for BZLF-1/AP-1 (denoted with a single asterisk), CREB (denoted with a double asterisk**), and SP1 (denoted with a triple asterisk***). These putative regulatory sequences are enclosed in boxes in the figure and include sequences recognized by the EBV encoded BZLF-1 regulatory protein (also termed ZEBRA protein). BZLF-1 sites also are also functional as sites for the endogenous regulatory factor AP-1 as discussed in the text. BZLF-1 regulated sites from other EBV genes ZIIIA, B, and ZRE1,2,3,5 are shown in comparison to a consensus AP-1 site in the lower portion of the figure. Similarly, in the lower portion of the figure the putative binding site for CREB is shown in the BALF-2 minimal promoter in comparison to Col8, a cAMP response element shown to bind CREB1 cAMP site binding protein with high affinity and ZII, a site in the BZLF-1 promoter shown experimentally to respond to cAMP. Also in the lower portion of the figure, a site in the BALF-2 minimal promoter matching the Sp1 transcription factor consensus is shown, similar but not identical to Sp1 binding sites confirmed to exist in the EBV terminal repeats (Figure 2).