. 2009 Mar 17;113(21):5134–5143. doi: 10.1182/blood-2008-11-190769

Table 2.

T cell subset characteristics

	CD4⁺ T-helper cells				CD8⁺ cytotoxic T cells
	Naive	Central memory	Effector memory	Effector	Naive	Central memory	Effector memory	Effector
CD62L	+	+	−	−	+	+	−	−
CD45RA	+	−	−	+	+	−	−	+
Changes from night to day^†, %	−41	−35	−32	NS	−46	−30	−23	23
Correlation with cortisol^‡	−0.65*	−0.62*	−0.54*	NS	−0.68*	−0.54*	−0.42*	NS
Changes after cortisol^§, %	−42	−38	−35	NS	−39	−27	−21	NS
GR expression^‖	1063 ± 31*	1098 ± 34***	1167 ± 35	1181 ± 36	1168 ± 33	1228 ± 50*	1280 ± 51**	1367 ± 55
Correlation with epinephrine^‡	NS	NS	NS	NS	NS	NS	NS	0.26*
Changes after epinephrine^§, %	NS	NS	NS	NS	NS	NS	NS	30
	Naive and central memory		Effector memory and effector		Naive and central memory		Effector memory	Effector
β-AR expression^¶	197 ± 42*		827 ± 239		727 ± 181**		2045 ± 579	2112 ± 602

Columns show data of naive (CD45RA⁺CD62L⁺), central memory (CD45RA⁻CD62L⁺), effector memory (CD45RA⁻CD62L⁻), and effector (CD45RA⁺CD62L⁻) CD4⁺ and CD8⁺ T cells.

NS indicates not significant.

^†

Percentage changes comparing the two extremes of the circadian rhythm during nighttime and daytime.

^‡

Correlations assessed by stepwise linear regression analyses between circadian variation in cortisol/epinephrine and respective T cell subpopulation, measured with a delay (time lag) of 3 and 0 hours with reference to cortisol and epinephrine, respectively. *P < .001.

^§

Percentage change in cell numbers 180 and 30 minutes after starting cortisol and epinephrine infusions, respectively, compared with placebo numbers (Figure 4I,J); n = 10.

^‖

Morning levels of GR expression in MFI, mean ± SEM; n = 8. *P < .05, **P < .01, ***P < .001, pairwise comparisons between naive versus central memory, central memory versus effector memory, and effector memory versus effector T cells, respectively.

^¶

Morning levels of β-AR expression measured by specific binding of ¹²⁵I-CYP in isolated T cells; data of saturation curves (Figure 5) were analyzed using nonlinear regression plot, and the maximum number of ¹²⁵I-CYP binding sites (B_max) is presented as mean ± SEM; n = 6. *P < .05, **P < .01, pairwise comparisons between CD62L⁺ versus CD62L⁻ T cells and effector memory versus effector CD8⁺ T cells, respectively.