Figure 1.

CXCR4 expression and cell-matrix adhesion among hematopoietic progenitors from Fancc^−/− mice and wild-type littermates. (A) Cell-surface expression of CXCR4 on lineage-depleted (lin⁻) and whole bone marrow (WBM) cells from C57Bl/6 strain mice. Assay carried out as described.⁵ Briefly, cells were harvested from Fancc^+/+ and Fancc^−/− (not shown) animals, samples were depleted of red cells by hemolysis and lineage-depleted using an Easy Sep Mouse Hematopoietic Progenitor Cell Enrichment kit (StemCell Technologies, Vancouver, BC) according to the manufacturer's instructions. Samples were stained with antibodies (fluorescein isothiocyanate [FITC] label) directed against surface CXCR4 epitopes or IgG (FITC; all BD Biosciences, San Jose, CA). Expression was analyzed using a FACSCalibur (BD Biosciences) and data were processed using FlowJo software (TreeStar, Ashland, OR). (B) Cell-matrix adhesion by WBM progenitors from Fancc^−/− and wild-type animals. The assay was performed using lineage-depleted progenitor cells generated as outlined above and was otherwise performed exactly as described by Zhang.¹