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Reconstitution of Ca2+-dependent nuclear accumulation of CaM in permeabilized cells. (a) The extent of import of fl-NLS-BSA into nuclei is unaffected by changes in free Ca2+. (b) The amount of fl-CaM found in the nucleus increases with increasing concentration of free Ca2+ in the import buffer. Note that cytoplasmic binding of fl-CaM is also a function of Ca2+ concentration. Bars represent 25 μm.
