Figure 3. Pax6-bound genes are enriched in genes required for nervous system development.

(A) Gene Ontology analysis for enrichment of categories of genes in the Pax6-bound set of genes. The set of Pax6-bound genes was compared to the whole genome to identify functional or biological sets over-represented in the Pax6-bound set, as described in the Materials and Methods. (B) Examples of genes in selected functional categories shown in A. (C,D) Cellular localisation of genes representative of some of the biological (C) and molecular (D) categories shown in (A), at E14.5 from the public in situ hybridisation database GenePaint [38]. The majority of genes are expressed in neocortical stem and progenitor cells in the ventricular zone (VZ) and basal progenitor cells in the subventricular zone (SVZ), with a significant minority also expressed in differentiating neurons in the cortical plate (CP). Scale bars, 500 µm.