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Effect of the molar ratio using 20 mg of enzyme at 60 °C.
| Molar Ratio (Acid/Amine) | Method Aa Yield | Method Bb Yield | MethodCc Yield |
| 1/0.33 | 11 | 12 | 12 |
| 1/0.5 | 11.5 | 12 | 12 |
| 1/1 | 87 | 92 | 97 |
| 1/2 | 11.5 | 12 | 12 |
| 1/3 | 11 | 12 | 12 |
aconventional method, bsolution phase method, csolid support method.
