Figure 2.

MBP-NBD purify as a mixture of different forms. (A) Amylose-purified MBP-NBD1 fractions were combined, concentrated and loaded onto a Superose 6 column (A, left) or a blue native gel (A, right). The void volume on the gel filtration chromatography is indicated by an arrow. The native gel was transferred onto a PVDF membrane and immunoblotted with anti-CTS antibody. (B) ATPase activities of the three fractions. Equal amounts of protein were assayed, containing the same amount of MBP-NBD1. The dotted line shows the level of activity associated with MBP alone.