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. 2009 Feb 17;2(4):221–231. doi: 10.1155/2009/280317

Figure 2.

Figure 2.

The pH, temperature and salt dependence of the Sulfolobus solfataricus recombinant WT amidase oligomerization process assessed by analytical centrifugation. Experiments were conducted at 117,000 g after overnight incubation at about 0.5 mg ml–1 at the indicated pH and temperature. Each trace refers to a different experiment. Experiments conducted at 4 (A), 20 (B) and 36 (C) °C in 20 mM sodium phosphate pH 6.0 (black), 7.0 (blue) and 8.0 (red). Experiments conducted in 20 mM sodium phosphate pH 6.0 (D), 7.0 (E) and 8.0 (F) at 4 (black), 20 (blue), and 36°C (red). Experiments conducted at 4 (G), 20 (H), and 36°C (I) in 20 mM sodium phosphate pH 6.0 in the presence (black) or in the absence (red) of 1 M NaCl.