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Turnover numbers (kcat, s–1) for Sulfolobus solfataricus WT amidase and Y41C determined at pH 5.0 and pH 8.0.
| Substrate | pH | WT | Y41C |
| Propionamide | 5.0 | 82.9 | 99.4 |
| 8.0 | 3.2 | 11.4 | |
| Isobutyramide | 5.0 | 48.9 | 58.4 |
| 8.0 | 21.9 | 40.8 | |
| Benzamide | 5.0 | 6.7 | 7.2 |
| 8.0 | 6.4 | 7.1 | |
| 3-Phenylpropionamide | 5.0 | 15.3 | 18.0 |
| 8.0 | 7.1 | 18.3 | |
