Figure 5.

Antibodies directed against eIF-5A block specifically the nuclear export of HIV-1 Rev and HTLV-I Rex NES. Comicroinjection of HeLa nuclei with the indicated export substrates and anti-eIF-5A antibodies (α-eIF-5A) (26). Cell were incubated for 20 min, fixed, and subjected to double-label, indirect immunofluorescence analysis. The GST-fusion proteins were visualized by using a mouse monoclonal anti-GST antibody followed by Cy3-conjugated goat anti-mouse IgG (GST). The microinjected rabbit antibodies directed against eIF-5A were detected with a Cy2-conjugated goat anti-rabbit antibody (IgG).